Ginfo.pl logo
  

YOU CAN NOW DISCUSS THIS OBJECT ON OUR FORUM JUST CLICK HERE!
Enzyme immunoassay for serum lidocaine in antiarrhythmic therapy.
People related with this object:

A L Levy
D P Lehane
Mark Kukucka
P Menyharth
P J Wissert
 
Object description
We have adapted the commercially available EMIT kit [Clin. Chem. 23, 1161 (1977)] to the Gilford System 3500 Analyzer. Sample volume is 10 microliter. We compare reagent blank-corrected absorbance changes at 340 nm between 15 and 55 s for samples and a series of calibrators, and calculate results with use of a logarithmic transformation. Within-run precision (CV) for a serum pool with 4.0 mg of added lidocaine per liter was 2.7% (n = 45); day-to-day precision was 3.3% (n = 15). Analytical recoveries of 2 to 6 mg of lidocaine per liter were 90-102% (average, 97.3%). Results correlated significantly with those by a gas-chromatographic technique. No clinically significant interferences by concomitantly administered medications were observed. The procedure is rapid (42 samples per hour) and is well suited to the fast response required in monitoring lidocaine therapy. Usefulness of the assay data in the management of arrhythmias in the coronary care unit is discussed.00099147Clin Chem. 1979 Apr ;25 (4):614-6
Created
0000-00-00 00:00:00
Comments
Hits
0